Zeiss Axioscan 7 - Slide Scanner

The Axioscan 7 is an automated slide scanner for brightfield, polarized light and fluorescence microscopy. Up to 100 slides can be loaded and scanned with 10x/0.45, 20x/0.45 Pol, 20x/0.8 or 40x/0.95 objectives.

High-quality virtual slides are created, that can be navigated, zoomed in, or shared. They can also be used for high throughput quantitative analysis.

Specifications

Imaging

Slide Scanner:

·         High-end brightfield

·         Circularly polarized light

·         Fluorescence

Detectors

Brightfield: AxioCam 705 (color)

Fluorescence: AxioCam 712 (monochrome)

Light Sources

Brightfield: WL-LED (400-700 nm, max at 460)

Fluorescence: Colibri.7 (385, 475, 555, 630, 735 nm)

Magazine

up to 100 slides

Software

Zeiss Zen Blue 3.5

Slide holders

25 holders for 4 slides 26x76 mm

2 holders for 2 slides 52x76 mm

2 holders for 1 slide 104x76 mm

Objectives

Objective

Mag. / NA

Working Distance

Plan Apochromat

10x / 0.45

2 mm

N-Achroplan cPol

20x / 0.45

0.63 mm

Plan Apochromat

20x / 0.8

0.55 mm

Plan Apochromat

40x / 0.95

0.25 mm

Light sources

LEDs

Type and Power

Some dyes and use

385nm

LED

DAPI, Hoechst, ...

475nm

LED

GFP, AlexaFluor488, Cy2

555nm

LED

RFPs, AlexaFluor568, Cy3

630nm

LED

DRAQ5, Cy5, AlexaFluor647

730nm

LED

Alexa750

Filter cubes for LEDs

Fluorochrome

Excitation

Dichroic

Emission

Filter No

Blue

BP365/12

FT395

LP397

01

Green

BP470/40

FT495

BP525/50

38HE

Red

BP550/25 (HE)

FT570

BP605/70

43HE

CFP/YFP/mCherry

370-400/494-528/583-600

BS450/538/610

458-474/546-564/518-756

91HE

Pentaband

370-400/450-488/540-570/614-647/720-750

410/493/575/654/761

412-438/501-527/582-601/662-700/770-800

112HE

Blue (DAPI)

BP335-383

BS395

BP420-470

49DAPI

 

Read more on the Axioscan 7 here.