LSM 900 & Airyscan2
The Zeiss LSM900 is a compact system delivering superb performance for routine fixed sample imaging using a laser scanning confocal. Mounted on an inverted microscope, it enables the scan live cells by using water immersion lenses, a stage-top chamber for environmental control and focus drift correction.
The system incorporates a smart, efficient detector disposition based on two variable beam splitters that drive the light to 3 detectors: 2 standard GaAsPs with high sensitivity across the spectrum, and the Airyscan2 detector. This is an array of 32 single GaAsP detectors arranged as a rosette which, in combination with the 40x / 1.3 objective, allows for super-resolution performance (down to 150 nm). Alternatively, this optical combination allows for use as a slit scanning confocal, increasing scanning speed by 8x.
Additionally, the system boasts an AxioCam 702 mono camera, enabling widefield acquisitions using the Colibri7 LED light source.
Specifications
|
Imaging |
Point, Array and Slit scanning confocal. |
|
Detectors |
2 GaAsPs for Fluorescence Airyscan2 detector: 32-channel GaAsP detector rosette 1 PMT for Transmitted Light AxioCam 702 mono camera for widefield |
|
Detection range |
From 405nm to 750nm |
|
Stand |
INVERTED Zeiss CellObeserver 7 |
|
Stage |
Motorized in XYZ |
|
Software |
Zeiss Zen Blue 3 |
|
Add. features |
DIC optics RGB-UV LED LED light source |
Objectives
|
Objective |
Mag. / NA |
Medium |
Contrast |
Working Distance |
| Fluar |
Air |
- |
8,7 mm |
|
|
Plan-Apochromat |
Air |
DIC II |
2,1 mm |
|
|
Plan-Apochromat |
Air |
DIC II |
0,55 mm |
|
|
Plan-Apochromat |
Oil |
DIC III |
0,13 mm |
|
|
Plan-Apochromat |
Oil |
DIC II HC |
0,19 mm |
Light Sources
|
Lasers |
Type and Power |
Some dyes and use |
|
405 nm |
Diode, 5 mW |
DAPI, Hoechst, AlexaFluor405, DyLight405 |
|
488 nm |
Diode, 10 mW |
GFP, AlexaFluor488, Cy2 |
|
561 nm |
Diode (SHG), 10 mW |
RFPs, AlexaFluor568, Cy3 |
|
639 nm |
Diode, 5 mW |
DRACQ5, Cy5, AlexaFluor647 |
|
Diodes Colibri7 Type R(G/Y)B-UV |
Main Beam Splitter
- 405/488/561/633
Airyscan filters
- Band pass defined by the variable beamsplitters
Filter Cubes for Colibri7 LED light source
|
Fluorochrome |
Excitation |
Dichroic |
Emission |
Filter No |
|
Blue |
BP390/40 |
FT420 |
BP450/40 |
|
|
Green |
BP470/40 |
FT495 |
BP525/50 |
|
|
Red |
BP545/25 |
FT570 |
BP605/70 |
|
|
DAPI, FITC, TRITC and Cy5 |
385, 475, 555 and 630 |
QBS 405 + 493 + 575 + 653 |
QBP 425/30 + 514/30 + 592/30 + 709/100 |
Applications
- Optical z-sectioning of fixed samples
- 3D-super-resolution imaging
- Fast confocal scanning of fixed samples
- Live cell imaging with stage-top environmental control (37C, CO2)
- Overlay of multiple fluorescence images with transmitted light images
- Widefield imaging of fixed samples