Axioscan.Z1: OPALS
The Axioscan is an automated slide scanner for brightfield and fluorescence microscopy, with filters suitable for Opal Fluorophores. Up to 100 slides can be loaded and scanned with 10x/0.45, 20x/0.8 or 40x/0.95 objectives.
High-quality virtual slides are created, that can be navigated, zoomed in, or shared. They can also be used for measurement and figure making.
Specifications
| Imaging |
Slide Scanner High-end brightfield Fluorescence (suitable for Opals) |
| Detectors |
Brightfield: CCD Hitachi HV-F202CLS Fluorescence: Axiocam 506 |
| Light Sources |
X-Cite CYLIS II XT730L - 385nm |
| Magazine | up to 100 slides |
| Software | Zeiss Zen Blue 3.5 |
| Slide holders |
25 holders for 4 slides 26x76 mm 1 holder for 2 slides 52x76 mm 2 holders for 1 slide 104x76 mm |
| Speed | 4 min for 15mmx15mm in Brightfield at 0.22um/px |
Objectives
| Objective | Mag. / NA | Working Distance |
| Plan Apochromat | 10x / 0.45 | 2 mm |
| Plan Apochromat | 20x / 0.8 | 0.55 mm |
| Plan Apochromat | 40x / 0.95 | 0.25 mm |
Light sources
X-Cite CYLIS II XT730L - 385nm
LED Peaks: 385, 430, 475, 545, 635, 735
Filters
|
Filter set |
Excitation |
Beamsplitter |
Emission |
|
Filter Set 122 SBP |
BP 390/19 |
BS 405 + 515 + 615 BS 465 + 550 + 690 + 770 |
BP 426/28 DBP 488/25 + 815/74 DBP 535/21 + 713/25 BP 571/20 BP 638/25 |
|
Fluorochrome |
Excitation |
Beamsplitter |
Emission |
|
DAPI/ Alexa Fluor 405 |
BP 380-399 |
BS 405 + 515 + 615 |
BP 475-500 & BP 778-852 |
|
Opal 480 / mCerulean / CFP |
BP 420-440 |
BS 465 + 550 + 690 + 770 |
BP 475-500 & BP 778-852 |
|
Opal 520 + 540 / Alexa Fluor 488 |
BP 490-507 |
BS 405 + 515 + 615 |
BP 524-545 & BP 700-725 |
|
Opal 570/ CY3 |
BP 522-542 & BP 734-758 |
BS 465 + 550 + 690 + 770 |
BP 561-581 |
|
Opal 620 / Opal 650/ Alexa Fluor 568/ TexasRed |
BP 588-604 |
BS 405 + 515 + 615 |
BP 625-650 |
|
Opal 690 |
BP 657-675 |
BS 465 + 550 + 690 + 770 |
BP 524-545 & BP 700-725 |
|
Opal 780 / Alexa Fluor 750 |
BP 522-542 & BP 734-758 |
BS 465 + 550 + 690 + 770 |
BP 475-500 & BP 778-852 |