Zeiss LSM 710
Laser scanning confocal microscopy has become the golden standard in imaging biological samples, allowing for good resolution and flexibility in stainings. LSM710 is an upright laser scanning confocal microscope. Its six laser lines and three detectors (including a spectral detector) make it a flexible microscope.
LSM710 is equipped with a dipping lens making it the system of choice for imaging cleared tissues.
Specifications
| Imaging | Point Scanning confocal |
| Detectors |
32-Array QUASAR detector 2 PMTs for Fluorescence |
| Detection | From 405nm to 750nm |
| Stand |
Upright Zeiss Axioimager Z2 |
| Stage | Motorized in XYZ |
| Software | Zeiss Zen Black 2012 |
Objectives
| Objective | Mag. / NA | Medium | Contrast | Working Distance |
| EC Plan-Neofluar | 10x/0.3 | Air | - | 5.2 mm |
| Plan-Apochromat | 20x/0.8 | Air | - | 0.55 mm |
| EC Plan-Neofluar | 40x/1.3 | Oil | DIC II | 0.21 mm |
| Plan-Apochromat | 63x/1.4 | Oil | DIC III | 0.19 mm |
| W N-Achroplan | 40x/0.75 | Water dipping | - | 2.1 mm |
Light sources
| Lasers | Type and Power | Some dyes and use |
| 405nm | Diode, 30mW | DAPI, Hoechst, ... |
| 458nm | Argon, 25mW | CFP |
| 488nm | Argon, 25mW | GFP, AlexaFluor488, Cy2 |
| 514nm | Argon, 25mW | YFP |
| 561nm | Solid State, 20mW | RFPs, AlexaFluor568, ... |
| 633nm | HeNe, 5mW | DRACQ5, Cy5, AlexaFluor648 |
Main Beam Splitters
| MBS 405 | MBS 458 |
| MBS 445 | MBS 458/514 |
| MBS 458/514/561/633 | |
| MBS 488 | |
| MBS 488/561 | |
| MBS 488/561/633 | |
| T80/R20 |
Filter cubes for Mercury lamp (HXP120)
| Fluorochrome | Excitation | Dichroic | Emission | Filter No |
| Blue | SP365 | FT395 | BP420-470 | 49 |
| Green | BP470/40 | FT495 | BP525/50 | 38HE |
| Red | BP550/25 (HE) | FT570 | BP605/70 | 43HE |
Applications
- Optical z-sectioning of living specimens and fixed samples,
- Spectral imaging and advanced unmixing of overlapping emission spectra,
- Overlay of multiple fluorescence images with transmitted light images,