Zeiss Axioscan 7 - Slide Scanner
The Axioscan 7 is an automated slide scanner for brightfield, polarized light and fluorescence microscopy. Up to 100 slides can be loaded and scanned with 10x/0.45, 20x/0.45 Pol, 20x/0.8 or 40x/0.95 objectives.
High-quality virtual slides are created, that can be navigated, zoomed in, or shared. They can also be used for high throughput quantitative analysis.
Specifications
Imaging |
Slide Scanner: · High-end brightfield · Circularly polarized light · Fluorescence |
Detectors |
Brightfield: AxioCam 705 (color) Fluorescence: AxioCam 712 (monochrome) |
Light Sources |
Brightfield: WL-LED (400-700 nm, max at 460) Fluorescence: Colibri.7 (385, 475, 555, 630, 735 nm) |
Magazine |
up to 100 slides |
Software |
Zeiss Zen Blue 3.5 |
Slide holders |
25 holders for 4 slides 26x76 mm 2 holders for 2 slides 52x76 mm 2 holders for 1 slide 104x76 mm |
Objectives
Objective |
Mag. / NA |
Working Distance |
2 mm |
||
20x / 0.45 |
0.63 mm |
|
0.55 mm |
||
0.25 mm |
Light sources
LEDs |
Type and Power |
Some dyes and use |
385nm |
LED |
DAPI, Hoechst, ... |
475nm |
LED |
GFP, AlexaFluor488, Cy2 |
555nm |
LED |
RFPs, AlexaFluor568, Cy3 |
630nm |
LED |
DRAQ5, Cy5, AlexaFluor647 |
730nm |
LED |
Alexa750 |
Filter cubes for LEDs
Fluorochrome |
Excitation |
Dichroic |
Emission |
Filter No |
Blue |
BP365/12 |
FT395 |
LP397 |
|
Green |
BP470/40 |
FT495 |
BP525/50 |
|
Red |
BP550/25 (HE) |
FT570 |
BP605/70 |
|
CFP/YFP/mCherry |
370-400/494-528/583-600 |
BS450/538/610 |
458-474/546-564/518-756 |
|
Pentaband |
370-400/450-488/540-570/614-647/720-750 |
410/493/575/654/761 |
412-438/501-527/582-601/662-700/770-800 |
|
Blue (DAPI) |
BP335-383 |
BS395 |
BP420-470 |
Read more on the Axioscan 7 here.