Abberior STEDYCON STED system
Awarded the Nobel Prize in Chemistry in 2014, STED is a super-resolution technique that enables visualizing your sample with 40 nm resolution. The STEDYCON system is a compact STED that boasts a powerful 775 nm depletion laser, enabling far-red and orange super-resolution imaging. Basically, the 775 nm laser creates a donut of light that narrows down the emission spot we create images with, hence increasing resolution. On top of that, the system has sensitive detectors that allow for 4-color confocal imaging (blue, green, orange/red, far red).
This microscope can also be used as an upright widefield microscope controlled by Zeiss' ZEN.
Specifications
| Imaging | STED + Widefield microscope |
| Detectors |
STEDYCON scanhead for confocal and super-resolution imaging, fitted with 4 APDs AxioCam 506 mono - 6Mpx B&W camera dedicated to widefield fluorescence imaging. |
| Stand |
Upright Zeiss AxioImager Z1 |
| Stage | Motorized in XYZ |
| Software |
Abberior Software suit for STED/Zeiss Zen Blue 2012 for widefield |
| Illumination | 405, 488, 561, 640 nm diode lasers for confocal and STED excitation, 775 nm STED depletion laser HXP120 for fluorescence Transmitted light |
| Filter cubes | Blue (DAPI, AlexaF405...) Green (FITC, AlexaF488...) Red (TRITC, ...) Triple Colour RGB |
| Contrast | Brightfield, darkfield |
Objectives
|
Objective |
Mag. / NA |
Medium |
Working Distance |
|
5x / 0.15 |
Air |
18.5 mm |
|
|
EC Plan-Neofluar |
10x / 0.3 |
Air |
5.2 mm |
|
20x / 0.5 |
Air |
2 mm |
|
|
63x / 1.4 |
Oil |
0.19 mm |
|
|
100x / 1.46 |
Oil |
0.11 mm |
Application
- STED 2-color super-resolution imaging (far-red/orange), with 40 nm resolution. Green long-Stoke shift dyes can also be used for super-resolution in the green part of the spectrum (80 nm). Special fluorophores required for all channels.