Zeiss LSM 700
Laser scanning confocal microscopy has become the golden standard in imaging biological samples, as it allows for good resolution and flexibility in stainings. LSM700 is a simple upright laser scanning confocal microscope optimal for imaging fixed triple stained (Blue, Green and Red / Far-red) samples.
Specifications
|
Imaging |
Point Scanning confocal |
|
Detectors |
2 PMTs for Fluorescence |
|
Detection |
From 405nm to 700nm |
|
Stand |
UPRIGHT Zeiss Axioimager M2 |
|
Stage |
Motorized in XYZ |
|
Software |
Zeiss Zen Black 2012 |
Objectives
|
Objective |
Mag. / NA |
Medium |
Contrast |
Working Distance |
| Plan-Neofluar | 5x / 0.15 | Air | - | 18.5 mm |
| EC Plan-Neofluar | 10x / 0.3 | Air | - | 5.2 mm |
| Plan-Apochromat | 20x / 0.8 | Air | - | 0.55 mm |
| EC Plan-Neofluar | 40x / 0.75 | Air | - | 0.71 mm |
| Plan-Apochromat | 63x / 1.4 | Oil | DIC | 0.19 mm |
Light sources
| Lasers | Type and Power | Some dyes and use |
| 405nm | Diode, 5mW | DAPI, Hoechst, ... |
| 488nm | Diode, 10mW | GFP, AlexaFluor488, Cy2 |
| 555nm | Diode, 10mW | AlexaFluor568 |
| 639nm | Diode, 5mW | DRACQ5, Cy5, AlexaFluor648 |
Main Beam Splitter
405/488/555/639
FILTER CUBES for Mercury lamp (HXP120)
| Fluorochrome | Excitation | Dichroic | Emission | Filter No |
| Blue | SP365 | FT395 | BP420-470 | 49 |
| Green | BP470/40 | FT495 | BP525/50 | 38HE |
| Red | BP550/25 (HE) | FT570 | BP605/70 | 43HE |