Olympus ScanR3 Spinning Disk

Inverted spinning disk confocal microscope designed for high-throughput content screening. Features fully automated image acquisition and data analysis. Equipped with hardware autofocusing, motorized focus and automatic XY stage.

Specifications

Imaging

Spinning disk confocal and widefield microscope

Detectors

Camera (Hamamatsu Orca flash 4) Chip: Effective number of pixels: 2048x2048 Pixel Size: 6.5μm

Stand

Inverted

Stage

Motorized in XYZ

Software

ScanR Acquisition cellSens

Add. features

Temperature and CO2 Control Fully automated screening microscope

Maerzhaeuser motorized stage (resolution 0.01μm, reproducibility 1.0μm, accuracy 3.0μm)

Autofocus: Hardwarefocus 785nm (ZDC)/Continuous ZDC and Softwarefocus

Multi Level Scan

 

Yokogawa CSU-W1Confocal spinning disk unit: Microlens array 50μmdisc

Objectives

Objective

Mag. / NA

Medium

WD

UPLXAPO 4x0.16

4x/0.16

Air

16

UPLXAPO 10x0.4

10x/0.4

Air

3.1

UPLXAPO 20x0.80

20x/0.8

Air

0.6

UPLXAPO 40x0.95

40x/0.95

Air

0.18

Light Sources

Lumencor Spectra LED´s

Lasers

Lasers

Type and Power

Some dyes and use

405 nm

Diode, 50 mW

DAPI, Hoechst, AlexaFluor405, DyLight405

488 nm

Diode, 100 mW

GFP, AlexaFluor488, Cy2

561 nm

Diode, 100 mW

RFPs, AlexaFluor568, Cy3

640 nm

Diode, 100 mW

DRACQ5, Cy5, AlexaFluor647

Fluorescent filter cubes

SPX-TSEM:

  • Triple band Fluorescence mirror unit
  • Triple band excitation filter and dichroic beamsplitter, three single band emission filters
  • Navy blue excitation (458 nm) for CFP and similar
  • Emerald excitation (515 nm) for YFP and similar
  • Orange excitation (594 nm) for mCherry and similar

SPX-QSEM:

  • Quad band Fluorescence mirror unit
  • Quad band dichroic beamsplitter, four single band emission filters
  • Violet excitation (<410 nm) for DAPI and similar
  • Blue excitation (480 nm) for EGFP, FITC and similar
  • Yellow excitation (560 nm) for RFP and similar
  • Red excitation (640 nm) for Cy 5 and similar

Applications

  • Fluorescence multichannel imaging of fixed and live cells
  • Fully automated image acquisition
  • High throughput screening
  • High content screening