STELLARIS 8 DIVE FALCON

The STELLARIS DIVE FALCON is an advanced multiphoton and confocal imaging system designed for deep-tissue, multicolor fluorescence imaging.

Equipped with the 4Tune spectrally tunable non-descanned detector, the system allows you to define up to four detection bands simultaneously—or an unlimited number when imaging sequentially—anywhere within the emission spectrum. This flexibility makes it easy to adapt to virtually any combination of fluorophores.

With STELLARIS DIVE, you can perform multiphoton imaging experiments with exceptional depth penetration, reaching beyond 1 mm, ideal for studying thick or living tissues. This capability enables detailed investigation of complex biological processes such as neuronal connectivity, organ architecture, dynamic cellular interactions, and spatial relationships between cells and proteins in vivo.

The FAst Lifetime COntrast (FALCON) module further enhances the system by enabling fluorescence lifetime imaging (FLIM). In addition to adding a new dimension of contrast through fluorescence lifetime information, this feature supports powerful applications for investigating cellular physiology and dynamics, biosensing, and protein interaction tracking.

Specifications

Imaging

Multiphoton + confocal + FLIM microscope

Scanning mode

Galvo Scanning and Resonant Scanning

Detectors

Confocal: Three Internal photon-counting HyD detectors

Multiphoton: Two 4Tune photon-counting Non Descanned Detectors (NDD); One PMT

Illumination

Confocal excitation: Diode Lasers 405nm, 488nm, 561nm, 638nm

Multiphoton excitation: SpectraPhysics InSight 3X+. Dual line: Tunable @ 680nm – 1300nm, Fixed line @ 1045nm.

Stage

Motorized in XYZ

Software

LAS X

Addition functions

NAVIGATOR – Multi-position, tile-scan module for large sample overviews

LIGHTNING – Real-time, deconvolution based, super-resolution module

TauSense – Integrated fluorescence lifetime–based imaging module for signal separation, background suppression, and functional contrast enhancement.

Objectives

Objective

Mag. / NA

Medium

Working Distances (in mm)

Comments

HC APO L 10x/0,30 W DLS

10x / 0.3

Air

3.6

HC IRAPO L 25x/1,00 W motCORR

25x / 1

Water

2.6

With or Without Coverslip

High End Obj

HC FLUOTAR L 25x/1,0 IMM (ne=1,457) motCORR VISIR

25x / 1

IMM (ne=1.457)

6

With or Without Coverslip

High End Obj

HC PL APO 40x/1,1 W CORR CS2

 40x /1.1 Water 0.62

Typical Applications

  • Optical z-sectioning and time-lapse imaging of living and fixed samples, including cultured cells, tissue explants, whole organs and etc.

  • Intravital imaging with awake and anesthetized animals with synchronized stimulations, pharmacology modulations and physiological monitoring

  • FRET/FRAP experiments

  • Label-free imaging, including SHG and THG

  • FLIM imaging with phasor plot analysis