Axiovert Z1

This basic, inverted Zeiss Axiovert Z1 microscope allows you to check your brightfield and fluorescent stainings by using either transmitted white light or LED-based fluorescence excitation (blue, green, red). Additionally, the system boasts phase contrast objectives, that can be used to introduce contrast into unstained samples. The microscope is fitted with an AxioCam 208 color camera, particularly suitable for brightfield imaging. This microscope is ideal if you want to take a few snapshots of your H&E or Sirius red staining, for example, or if you just want to check if your fluorescent staining worked. The phase contrast objectives are ideal to look at unlabeled Prokaryotic or Eukaryotic cells on culture vessels, since the microscope boasts an inverted stand. This microscope does not need to be booked when checking samples by eye, but please do if you will be taking images with it.

Imaging

Brightfield + Pase Contrast + Fluorescence

Detectors

AxioCam 208 - 8 Mpx 4k CMOS color camera dedicated to Brightfield imaging.

Stand

Inverted

Zeiss Axiovert Z1

Stage

Manual in XYZ

Software

Zeiss Zen Blue Lite for camera control

Illumination

White light for Brightfield and Phase contrast (transmitted light)

Blue/Green/Red LED for fluorescence

Filter cubes

01 BFP (DAPI, blue)

38 GFP (green)

43 RFP (Cy3, red)

Contrast

Brightfield, Phase contrast

Objectives

Objective

Mag. / NA / Phase

Medium

N- AchroPlan

5x / 0.15 / Ph1

Air

N- AchroPlan

10x / 0.25 / Ph1

Air

LD-PN

20x / 0.4 / Ph2

Air

Plan Apo

20x / 0.8

Air

LD-PN

40x / 0.6 / Ph2

Air

Application

  • Brighfield imaging: H&E, Sirius red, etc.
  • Phase contrast: Prokaryotic or Eukaryotic unlabeled cells grown on vessel. 
  • Widefield microscopy: Metal halide lamp and filter-based microscope for checking green/red stainings.