Comparison of SIM (left) and Widefield (right) images of the same structure.
Zeiss Elyra 7 Super Resolution Microscope
Elyra is a super resolution microscope with Structured Illumination Microscopy (SIM), Total Internal Reflection Fluorescence (TIRF) and Single Molecule Localization Microscopy (PALM or dSTORM for example).
Structured Illumination Microscopy (SIM) relies on light interfences creating Moiré fringes and improves the resolution by a factor of 2 (xy: 100nm; z: 250nm). Here are some recommendations for sample preparation for SIM. In burst mode, the system can achieve speeds of up to 250 fps. The system is also capable of doing a dedicated deconvolution process, SIM2, that enhances further the SIM resolution.
Localization microscopy techniques such as dSTORM and PALM take advantage of the blinking properties of some fluorophores to calculate their precise location in a sample. The resolution is greatly improved (xy: 40nm ; z: 100nm via TIRF).
Apotome and TIRF imaging are also possible in this system.
The system boasts full incubation chamber for live cell imaging, as well as DualCam for 2-color simultaneous imaging.
Specifications
| Imaging | Widefield / SIM / SIM2 / Apotome / PALM / dSTORM / TIRF |
| Cameras |
2x sCMOS PCO.edge 4.2 |
| Stand |
INVERTED Zeiss AxioObserver with incubation chamber |
| Stage | Motorized in XY, piezo insert in Z |
| Software | Zeiss Zen Black 2012 |
Objectives
| Objective | Mag. / NA | Medium | Contrast | Working Distance |
| EC Plan Neofluar | 10x / 0.3 | Air | DIC | 5.2 mm |
| Plan-Apochromat | 20x / 0.8 | Air | DIC | 0.55 mm |
| Plan-Apochromat | 40x / 1.2 | Water | DIC | 0.28 mm at 0.17 |
| Plan-Apochromat | 63x / 1.4 | Oil | DIC | 0.19 mm |
| alpha Plan-Apochromat | 63x /1.46 | Oil | - | 0.11 mm |
Light sources
| Lasers | Type and Power | Some dyes and use |
| 405 | HR Diode - 50mW | DAPI, Hoechst, AlexaFluor405... |
| 488 | HR Diode -500mW | GFP, AlexaFluor488... |
| 561 | HR Diode -500mW | RFPs, AlexaFluor555, 568... |
| 642 | HR Diode -500mW | AlexaFluor647, DRACQ5, Cy5... |
Filter sets for Super Resolution
| MBS | Emission | Fluorophores |
| 405/488/561/642 | LBF405/488/561/642 | All |
| 405/488/561/642 | BP420-480/BP495-550/LP650 | Blue + Green + Far Red |
| 405/488/561/642 | BP495-550/BP570-620 | Green + Red |
| 405/488/561/642 | BP420-480/LP655 | Blue + Far Red |
| SBS | Camera 1 | Camera 2 |
| LP560 | Red + Far Red | Blue + Green |
| BP490-560/LP640 | Green + Far Red | Blue + Red |
Filter cubes for HXP lamp
| Fluorophores | Excitation | Dichroic | Emission | Filter No |
| Green/Red/Far Red | TBP 483+564+642 | TFT 506+582+659 | TBP 526+601+688 | 77HE |