Leica AFS2 and FSP
Automatic freeze substitution with processor
Photo credit: Leica
Freeze Substitution (FS) of specimens in methanol, acetone or any other FS media at low temperatures is THE follow-on procedure to high pressure freezing and other Cryo fixation methods.
Progressive Lowering of Temperature (PLT) allows substituting and resin infiltratration of chemically fixed specimens.
Sample may be polymerized with UV light.
The Freeze Substitution Processor (FSP) is an automatic reagent handling system. Mounted on the AFS₂ it dispenses reagents for both FS and PLT. The FSP automatically dilutes FS media and resins from 100% reagent containers.
An integrated LED UV-lamp allows polymerization of the samples under cryo conditions.
CFIM
