Consumables and Reagents

Consumables for EM

Price DKK
Negative staining carbon grids per 10 200
Cryo TEM carbon grid per 10 200
Cryo TEM quantifoil grid per 10 1000
Thermanox coverslips Ø 13 mm per 5 50
Sapphire discs 6 mm per disc 220
Protein A-Gold 10 nm per 5 µl 75
Protein A-Gold 5 nm per 5 µl 75
Autogrid ring per 10 1000
C-Clip per 10 150
3 mm Carriers for HPF or Freeze fracture per 1 20
6 mm Carriers for HPF or Freeze fracture per 1 30


Consumables for LM

Antibodies

Item Vendor Catalog # Aliquot size (µl) Price DKK
donkey anti-goat AF 488 secondary antibody Invitrogen A11055 10 150
donkey anti-rabbit AF 488 secondary antibody Invitrogen A21206 10 150
donkey anti-rat AF 568 secondary antibody Invitrogen A78946 10 150
goat anti-mouse AF 488 secondary antibody Invitrogen A11029 10 150
goat anti-mouse AF 568 secondary antibody Invitrogen A11031 10 150
goat anti-mouse AF 647 Plus secondary antibody Invitrogen A32728 10 150
goat anti-mouse AF 647 secondary antibody Invitrogen A21236 10 150
goat anti-mouse STAR orange secondary antibody Abberior STORANGE-1001-500UG 25 200
goat anti-mouse STAR red secondary antibody Abberior STRED-1001-500UG 25 200
goat anti-rabbit AF 488 secondary antibody Invitrogen A11034 10 150
goat anti-rabbit AF 568 secondary antibody Invitrogen A11036 10 150
goat anti-rabbit AF 647 Plus secondary antibody Invitrogen A32733 10 150
goat anti-rabbit AF 647 secondary antibody Invitrogen A21245 10 150
goat anti-rabbit STAR 460L secondary antibody Abberior ST460L-1002-500UG 25 200
goat anti-rabbit STAR orange secondary antibody Abberior STORANGE-1002-500UG 25 200
goat anti-rabbit STAR red secondary antibody Abberior STRED-1002-500UG 25 200
goat anti-rat STAR red secondary antibody Abberior STRED-1007-500UG 25 200


Specific stains

Item Vendor Catalog # Stock solution Suggested work dilution
Aliquot size (µl)		 Price DKK
lysotracker Red DND-99 Invitrogen L7528 1mM in DMSO 50-75 nM 10 150
MitoTracker Orange CMTMRos Invitrogen M7510 1mM in DMSO 25–500 nM 10 150
MitoTracker Red CM-H2XRos Invitrogen M7513 1mM in DMSO 25–500 nM 10 150
phalloidin AF 555 Invitrogen A30106 400x stock solution in DMSO 0.5 µl of stock solution in 200 µl of PBS for each coverslip 10 350
phalloidin AF 647 Invitrogen A30107 400x stock solution in DMSO 0.5 µl of stock solution in 200 µl of PBS for each coverslip 10 350
SYTOX Deep Red nucleic acid stain Invitrogen S11381 5mM stock solution in DMSO 0.1–5 µM 20 400
SYTOX Green nucleic acid stain Invitrogen S7020 5mM stock solution in DMSO 0.1–5 µM 20 400
SYTOX Orange nucleic acid stain Invitrogen S11368 5mM stock solution in DMSO 0.1–5 µM 20 400
WGA 488 Invitrogen W11261 1mg/mg stock solution 5 µg/ml in HBSS 200 300
WGA 647 Invitrogen W32466 1mg/mg stock solution 5 µg/ml in HBSS 200 300

Mounting media

Item Vendor Catalog #
Aliquot size (µl)		 Price DKK
mounting medium Abberior Mount Solid Abberior MM-2011-2x15ML 150 25
mounting medium Prolong Gold Invitrogen P36934 50 25
mounting medium Slowfade Gold Invitrogen S36936 50 25
mounting medium Vectashield Vector Laboratories VECTVEC-H-1000 50 25

Laser dissection

Item Vendor Catalog #
Aliquot size (µl)		 Price DKK
adhesive cap tube 200 Zeiss 415190-9191-000 1 tube 50
adhesive cap tube 500 Zeiss 415190-9211-000 1 tube 70
membrane coated slides 0.17 PEN Zeiss 415190-9061-000 1 slide 45
membrane coated slides 1.0 PEN Zeiss 415190-9042-000 1 slide 45

Slids / Coverslips / Dishes

Item Vendor Catalog # Quantity Price DKK
Coverslips 18x18, #1.5H Marienfeld 0107032 200 200
Coverslips 22x22, #1.5 VWR 631-0125 200 100
Coverslips 22x50, #1.5 VWR 631-0138 100 100
Coverslips 24x24, #1.5 VWR 630-2106 200 100
Coverslips 24x32, #1 VWR 631-0144 100 100
Coverslips 24x50, #1.5 VWR 631-0147 100 100
Coverslips 24x60, #1 Marienfeld 10076371 100 100
Coverslips 25x75, #1.5 Thermo Scientific 100 200
Coverslips Ø 12, #1.5H Marienfeld 0117520 100 250
Coverslips Ø 18, #1.5H Marienfeld 630-2200 100 350
Coverslips Ø 30, #1.5 Thermo Scientific 100 150
Coverslips Ø 30, #1.5 Epredia CB00300RAC40MNZ0 100 150
Coverslips Ø 32, #1.5 Thermo Scientific 100 250
Coverslips Ø 42, #1.5H Thermo Scientific 100 250
Superfrost slides VWR 631-0108 10 50
Microscope slides VWR 631-1163 50 50
plate, 24-well ibidi 82406 1 250
plate, 384-well ibidi 88416 1 250
plate, 96-well ibiTreat ibidi 89626 1 250
plate, 96-well uncoated ibidi 89621 1 250
3-well chamber, removable ibidi 80381 1 150
8-well chamber, removable ibidi 80841 1 150
u-slide, 4-well ibidi 80426 1 150
u-slide, 8-well ibidi 80826 1 150
u-slide angiogenesis, 15 well 3D ibidi 81506 1 150
u-slide VI 0.4 ibidi 80606 1 150
u-dish with grid-500, glass bottom ibidi 81168 1 100
u-dish with grid-500, polymer ibidi 81166 1 50
u-dish Ø 35 mm 1.5H glass bottom ibidi 81158 1 50
u-dish Ø 35 mm, culture-insert, 2 well, ibiTreat ibidi 81176 2 200
u-dish Ø 35 mm, ibiTreat ibidi 81156 5 200
glass base dish Ø 35 mm, Ø 12 mm glass Thermo Scientific 150680 1 100
8-well chamber slide, Lab-Tech Thermo Scientific 177402 1 150
8-well chamber slide, Lab-Tech CC2 Thermo Scientific 154941 1 250

Chemicals

Item Quantity Price DKK
Ethyl cinnamate (ECi) 100 ml 200
Dichloromethane (DCM) 100 ml 500
PFA 16%, methanol free 10 ml 159

Other

Item Quantity Price DKK
Glass vial, 14 ml 1 11
Glass vial, 28 ml 1 14
128 GB USB drive 1 200