LM Sample preparation
Access to pathology lab
Histology services including conventional histology, immunohistochemistry and morphometric analysis can be provided by Histolab for a fee.
The services offered include:
- Conventional histology
- Immunohistochemistry
- Morphometric analysis
Histolab is located at the Department of Biomedical Sciences and run by Associate Professors Jens Christian Brings Jacobsen & Steen Seier Poulsen and Biomedical Laboratory Technologists Heidi Paulsen & Bente Stærgaard.
LM prep equipment
CFIM offers access to a cell culture facility and additional equipment, useful for sample preparation.
Vibrating microtome that uses a high-frequency oscillating cutting blade, combined with a slight compression that leads to consistent tissue sections (in fixed or acute slices).
The vibrating head is particularly designed to eliminate vibrations in the z-axis, reducing damage to surface cells on live tissue samples and further reducing chatter marks on thin sections for improved imaging results.
Ideal for relatively thick sections of tissue (30-1000 µm).
Technical specifications
Samples
- Fixed tissues
- Acute slices/Unfixed tissue
- Organoids
- Organotypic slice cultures
Applications
- Immunohistochemistry
- Live imaging of acute slices
- Light microscopy
- Electron Microscopy
- Electrophysiology
Links of interest
https://precisionary.com/tissue-sectioning-help/how-is-compresstome-different/#101-c
State of the art cryostat with quick freezing shelf.
Specifications |
|
| Section thickness | 0.5 to 300 μm |
| Max specimen size | 40 x 55 mm |
| Chamber cooling | 0°C to -40°C |
Description of the Wet Cell Prep Lab
A GMO1 certified Wet Cell Prep Lab is available at CFIM for final experimental steps before imaging.
You need to be authorized to use the Wet Cell Prep Lab, so please contact staff members prior to use.
The laboratory is equipped with
- Small fridge
- Small -20ºC freezer
- Safety cabinet - Thermo SAFE 2020
- Incubator with temperature and CO2 control
- Bench top centrifuge with cooling and warming
- Vacuum System - for suction and disposal of liquids.
- Pipettes
- Water bath
- Vortex
- Magnetic stirrer
- Mini-centrifuge