Light microscopy - Course for CFIM users - Aug 2012

This course is aimed at CFIM users, who want to understand the basic principles of optics and fluorescence in microscopy. Theoretical lectures are followed by practicals.


Basic Principles of microscopy

- Optics of the microscope
- Diffraction, Resolution and Contrast

Fluorescence and Confocal Microscopy

- Fluorescence microscopy (fluorophores, illumination…)
- Basic Confocal microscopy (Lasers, multi-dimensional Image Recording …)