LM sample preparation – University of Copenhagen

CFIM > Services > LM sample preparation

LM sample preparation

Histology services including conventional histology, immunohistochemistry and autoradiography can be provided by Steen Seier Poulsen laboratory for a fee. Contact Steen Seier Poulsen prior to sending any sample. All samples should be accompanied by a submission form.

The services offered include:

Conventional histology:

Preparation and examination of human tissues and tissues from experimental animals including investigation of the organs from genetic modified animals.
Trimming and parrafin embedding of tissues, sectioning and staining. Standard stainings are hematoxylin – eosin (HE), periodic-acid-shiff (PAS) for glycoproteins and mucus, and Sirius red primarily for collagen identification.
Other more specialised stainings and decalcification of tissues are also available
See standard staining examples

Immunohistochemistry:  

Identification of receptor proteins or peptides in histologic sections by means of specific antibodies raised against the target proteins. The histolab has available a large selection of in-house antibodies but has also an extensive experience in testing and characterization of new antibodies. Immunoreactions can be visualized for bright field microscopy or flourescence microscopy.
Researchers not familiar with evaluation of immunoreactions can get assistance for the evaluation of the stainings.
See Immunohistochemistry examples

Autoradiography: 

Radioactive tracers – usually iodinated with I125 can be visualized by autoradiography. This is especially suitable to identify receptorbinding. The tissue localisation of radioactive tracers given intravenously to experimental animals can be visualized in histologic sections of tissues exposed to the tracer. The sections are covered with a fluid photographic film, stored in darkness for some weeks and developed. The emission from the tracer will be seen as black dots on the slide.
See Autoradiography examples

Morphometric analysis:

Assistance with quantification of the occurrence of tissue components in histologic slides such as collagen (fibrosis), number of immunoreactive cells (endocrine cells, inflammatory cells, cells in mitosis etc), and dimensions of structures (intestinal villus height, epithelial thickness etc) using the image analysis program “Image Pro Plus”.

For cases which need extensive, specific interpretation, in particular in a scientific context, which are not part of collaborative work involving pathologists, additional consultancy charges will apply (475DKK/€50/hour).

We take no responsibility for new antibodies that we test, you will get charged even if we don’t get expected results.

When testing on “in house” antibodies, we can always guaranty positive results on our own positive control tissue.

Please contact CFIM-HIST prior to submission: spoulsen@sund.ku.dk

Please use the Sample Submission Form and submit your material to:
Københavns Universitet
Biomedicinsk Institut – CFIM
Steen Seier Poulsen
Symbion Science Park
Fruebjergvej 3
2100 København Ø