LSM 710 – University of Copenhagen

Zeiss LSM 710

Laser scanning confocal microscopy has become the golden standard in imaging biological samples, allowing for good resolution and flexibility in stainings. LSM710 is an upright laser scanning confocal microscope. Its six laser lines and three detectors (including a spectral detector) make it a flexible microscope.
LSM710 is equipped with a dipping lens making it the system of choice for imaging cleared tissues.

       Imaging Point Scanning confocal

32-Array QUASAR detector

2 PMTs for Fluorescence
1 PMT for Transmitted Light

Detection From 405nm to 750nm


Zeiss Axioimager Z2

Stage Motorized in XYZ
Software Zeiss Zen Black 2012

Objective Mag. / NA Medium Contrast Working Distance
EC Plan-Neofluar 10x/0.3 Air - 5.2 mm
Plan-Apochromat 20x/0.8 Air - 0.55 mm
EC Plan-Neofluar 40x/1.3 Oil DIC II 0.21 mm
Plan-Apochromat 63x/1.4 Oil DIC III 0.19 mm
W N-Achroplan 40x/0.75 Water dipping - 2.1 mm

Lasers Type and Power Some dyes and use
405nm Diode, 30mW DAPI, Hoechst, ...
458nm Argon, 25mW CFP
488nm Argon, 25mW GFP, AlexaFluor488, Cy2
514nm Argon, 25mW YFP
561nm Solid State, 20mW RFPs, AlexaFluor568, ...
633nm HeNe, 5mW DRACQ5, Cy5, AlexaFluor648

Main Beam Splitters  
MBS 405 MBS 458
MBS 445 MBS 458/514
MBS 458/514/561/633
MBS 488
MBS 488/561
MBS 488/561/633

FILTER CUBES for Mercury lamp (HXP120)
Fluorochrome Excitation Dichroic Emission Filter No
Blue SP365 FT395 BP420-470 49
Green BP470/40 FT495 BP525/50 38HE
Red BP550/25 (HE) FT570 BP605/70 43HE


Optical z-sectioning of living specimens and fixed samples,

Spectral imaging and advanced unmixing of overlapping emission spectra,
Overlay of multiple fluorescence images with transmitted light images,

More about LSM 710