Widefield – University of Copenhagen

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CFIM > Equipment > Light microscopes > Widefield

Zeiss Widefield microscope

Zeiss widefield microscope is an upright microscope. Samples can be illuminated with either fluorescent (for immunofluorescence stainings) or trasnmitted light (HE stainings for example). Samples can be viewed by up to three observers at the same time (conversation microscope) or captured with the CCD camera.   

Booking and use

The microscope is also available as a free of charge “Drop-In” service (no booking) to check samples without recording images (i.e. no logging on the computer, no use of the camera). Please remember that users who booked have first priority. In all cases, an introduction to the system is required before you use it the first time. You can book Zeiss WF via the online booking system.

 SPECIFICATIONS
Imaging Wide field microscope
Detectors

AxioCam 506 mono - 6Mpx B&W camera dedicated to fluorescence imaging.

AxioCam MRc5 - 5Mpx Colour camera dedicated to brightfield imaging.

Stand

Upright

Zeiss AxioImager Z1

Stage Motorized in XYZ
Software

Zeiss Zen Blue 2012

Illumination HXP120 for fluorescence
Transmitted light
Filter cubes Blue (DAPI, AlexaF405...)
Green (FITC, AlexaF488...)
Red (TRITC, ...)
Triple Colour RGB
Contrast Brightfield, darkfield

OBJECTIVES
Objective Mag. / NA Medium Working Distance
EC Plan-Neofluar 10x / 0.3 Air 5.2 mm
EC Plan-Neofluar 5x / 0.15 Air 18.5 mm
EC Plan-Neofluar 20x / 0.5 Air 2 mm
EC Plan-Neofluar 40x / 0.75 Air 0.71 mm
Plan-Apochromat 63x / 1.4 Oil 0.19 mm
LD Plan Neofluar 40x / 0.6 Air depends of coverslip thickness

APPLICATIONS

Widefield microscopy: Arc lamp and filters based microscope allowing the specific excitation and recording of classical fluorophores (Blue, green, red and far-red emissions).

Brightfield: transmitted illumination for non-fluorescent stainings such as HE, ISH...
Darkfield: Contrast technique based on scattering from the sample. Typically used with unstained (transparent) samples.