LSM 780 – University of Copenhagen

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CFIM > Equipment > Light microscopes > LSM 780

FRET by photobleaching of the acceptor. Upon photobleaching of Cx43-YFP (right), the intensity of Cx43-CFP increases (left).

Zeiss Confocal microscope LSM 780

Laser scanning confocal microscopy has become the golden standard in imaging biological samples, allowing for good resolution and flexibility in stainings. 

LSM780 is an inverted laser scanning confocal microscope equipped for live imaging (temperature and CO2 control). It has a tunable laser giving it a high flexibility in fluorophore excitation. The GaASP detector allows for detection of faint stainings and spectral imaging (separation of spectrally overlapping fluorophores). 

 SPECIFICATIONS
  Imaging Point Scanning confocal
                 Detectors

32-channels GaAsP detector

2 PMTs for Fluorescence (PMT2 is cooled)
1 PMT for Transmitted Light

Detection From 405nm to 750nm
Stand

INVERTED 

Zeiss AxioObserver Z1

Stage Motorized in XYZ
Software Zeiss Zen Black 2012
 Add. features Incubator (temperature + gas control) for live cell imaging

OBJECTIVES
Objective Mag. / NA Medium Contrast Working Distance
EC Plan-Neofluar 10x / 0.3 Air - 5.2 mm
LD Plan Neofluar 20x / 0.8 Air - Adjustable
LD Plan Neofluar 40x / 0.6 Air - Adjustable
C Apochromat 40x / 1.2 Water - Adjustable
EC Plan-Neofluar 40x / 1.3 Oil DIC II 0.21 mm
Plan-Apochromat 63x / 1.4 Oil DIC III 0.19 mm

LIGHT SOURCES
Lasers Type and Power Some dyes and use
405nm Diode, 30mW DAPI, Hoechst, ...
458nm Argon, 25mW CFP
488nm Argon, 25mW GFP, AlexaFluor488, Cy2
514nm Argon, 25mW YFP
543nm HeNe, 1.2mW RFPs, AlexaFluor568
633nm HeNe, 5mW DRACQ5, Cy5, AlexaFluor647
Intune 488 - 645nm 5mW

MAIN BEAM SPLITTERS
MBS 405/490c/640c MBS 458
MBS 405/505c MBS 458/514
MBS 405/520c MBS 458/543
MBS 405/535c MBS 488
MBS 405/550c MBS 488/543
MBS 405/565c MBS 488/543/633
MBS 405/580c T80/R20
MBS 405/595c
MBS 405/610c
MBS 405/625c

FILTER CUBES for Mercury lamp (HXP120)
Fluorochrome Excitation Dichroic Emission Filter No
Blue SP365 FT395 BP420-470 49
Green BP470/40 FT495 BP525/50 38HE
Red BP550/25 (HE) FT570 BP605/70

43HE

APPLICATIONS

Optical z-sectioning of living specimens and fixed samples,

FRET/FRAP/FLIP/FLIM experiments,
Photoactivation and photoconversion experiments,
Spectral imaging and advanced unmixing of overlapping emission spectra,
Overlay of multiple fluorescence images with transmitted light images,
Fluorescence Correlation Spectroscopy (FCS) (up to 6 channels),
Raster Image Correlation Spectroscopy (RICS).

 

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