LSM 700 – University of Copenhagen

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CFIM > Equipment > Light microscopes > LSM 700

Zeiss LSM 700

Laser scanning confocal microscopy has become the golden standard in imaging biological samples, as it allows for good resolution and flexibility in stainings. LSM700 is a simple upright laser scanning confocal microscope optimal for imaging fixed triple stained (Blue, Green and Red / Far-red) samples.

SPECIFICATIONS
Imaging Point Scanning confocal
Detectors 2 PMTs for Fluorescence
1 PMT for Transmitted Light
Detection From 405nm to 700nm
Stand UPRIGHT Zeiss Axioimager M2
Stage Motorized in XYZ
Software Zeiss Zen Black 2012

 

OBJECTIVES    
Objective Mag. / NA Medium Contrast Working Distance
Plan-Neofluar 5x / 0.15 Air - 18.5 mm
EC Plan-Neofluar 10x / 0.3 Air - 5.2 mm
Plan-Apochromat 20x / 0.8 Air - 0.55 mm
EC Plan-Neofluar 40x / 0.75 Air - 0.71 mm
Plan-Apochromat 63x / 1.4 Oil DIC 0.19 mm

 

LIGHT SOURCES
Lasers Type and Power Some dyes and use
405nm Diode, 5mW DAPI, Hoechst, ...
488nm Diode, 10mW GFP, AlexaFluor488, Cy2
555nm Diode, 10mW AlexaFluor568
639nm Diode, 5mW DRACQ5, Cy5, AlexaFluor648

 

Main Beam Splitter
405/488/555/639

 

FILTER CUBES for Mercury lamp (HXP120)
Fluorochrome Excitation Dichroic  Emission  Filter No
Blue SP365 FT395 BP420-470 49
Green BP470/40 FT495 BP525/50 38HE
Red BP550/25 (HE) FT570 BP605/70 43HE

 

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