Laser Microdissection – University of Copenhagen

Forward this page to a friend Resize Print Bookmark and Share

CFIM > Equipment > Light microscopes > Laser Microdissection

Laser Microdissection

Microdissection allows one to select and isolate part of a sample and use it for further studies (RT-PCR, protein analysis, selection of live cells). Laser microdissection can also be used with live samples to study recovery after a cut, protein recruitment after photodamage… The system is paired with optical tweezer to manipulate samples.

This widefield microscope is equipped with:

  • 3 sets of filters for imaging (Blue/Green/Red)
  • a laser microdissection unit - dissection and recovery of cells or subcellular compartments,
  • optical tweezers - mechanical manipulation of cells or samples without contact by using an infra-red laser,
  • an incubator for live cell imaging (time lapse).
SPECIFICATIONS
Imaging Widefield
Specificity Laser Microdissection
Optical Tweezers
Cameras AxioCam Icc1 version3
AxioCam MRm version3
Stand

INVERTED 

Zeiss Axio Observer Z1

Stage Motorized in XYZ
Software Robo software 4.2 Pro
Incubator Incubator (temperature + gas control) XL PALM S1 system

OBJECTIVES Objective markings
Objective Mag. / NA Medium Contrast Working Distance
Fluar 5x / 0.3 Air - 12.5 mm
LD Plan Neofluar 20x / 0.4 Air - Adjustable
LD Plan Neofluar 40x / 0.4 Air - Adjustable
Plan-Neofluar 63x / 0.75 Oil - Adjustable
Plan-Apochromat 63x / 1.4 Oil DIC III 0.19 mm
Plan Neofluar 100x / 1.3 Oil - 0.2 mm

FILTER CUBES for Mercury lamp (HXP120)
Fluorochrome Excitation Dichroic Emission Filter No
Blue SP365 FT395 BP420-470 49
Green BP470/40 FT495 BP525/50 38HE
Red BP550/25 (HE) FT570 BP605/70

43HE

SUGGESTED REFERENCES
Laser microdissection
  • Isolation of RNA
Pedersen et al., 2005
  • Proteomics
Mukherjee et al., 2013
  • Isolation of live cells
Mustafa et al., 2012
  • Effect of dissection on Live cells
Fink et al., 2011
Optical tweezers
  • Review
van Mameren et al., 2011
  • Force measurements
Jannasch et al., 2013

PROTOCOLS from Zeiss

RNA handling

RNA extraction from frozen sections
RNA extraction from FFPE sections

RNA Quality

DNA handling
Chromosome preparation
Protein handling
Cell recultivation
Immunofluorescence on frozen sections
FISH hybridization

Quick guide to the PALM ROBOsoftware